fcv strain f 9 Search Results


fcv attenuated vaccine strain f9  (Intervet International GmbH)
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Intervet International GmbH fcv attenuated vaccine strain f9
Specificity of the triplex assay. FPV, <t>FCV,</t> <t>FHV-1,</t> and other animal pathogens (including FIV, FeLV, FCoV, RV, and PRV) were selected and tested using the triplex assay. Only FCV, FHV-1, and FPV detected positive.
Fcv Attenuated Vaccine Strain F9, supplied by Intervet International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Specificity of the triplex assay. FPV, FCV, FHV-1, and other animal pathogens (including FIV, FeLV, FCoV, RV, and PRV) were selected and tested using the triplex assay. Only FCV, FHV-1, and FPV detected positive.

Journal: Frontiers in Veterinary Science

Article Title: Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1

doi: 10.3389/fvets.2021.792322

Figure Lengend Snippet: Specificity of the triplex assay. FPV, FCV, FHV-1, and other animal pathogens (including FIV, FeLV, FCoV, RV, and PRV) were selected and tested using the triplex assay. Only FCV, FHV-1, and FPV detected positive.

Article Snippet: FCV (attenuated vaccine strain F9), FHV-1 (attenuated vaccine strain G2620A), and FPV (attenuated vaccine strain MW-1) were purchased from Intervet International B.V. Wild strains of these three viruses were isolated and stored by our laboratory, and the virus titers were 10 8.0 TCID 50 /0.1 ml for FCV, 10 7.0 TCID 50 /0.1 ml for FPV, and 10 8.3 TCID 50 /0.1 ml for FHV-1.

Techniques:

Sensitivity of the real-time PCR.

Journal: Frontiers in Veterinary Science

Article Title: Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1

doi: 10.3389/fvets.2021.792322

Figure Lengend Snippet: Sensitivity of the real-time PCR.

Article Snippet: FCV (attenuated vaccine strain F9), FHV-1 (attenuated vaccine strain G2620A), and FPV (attenuated vaccine strain MW-1) were purchased from Intervet International B.V. Wild strains of these three viruses were isolated and stored by our laboratory, and the virus titers were 10 8.0 TCID 50 /0.1 ml for FCV, 10 7.0 TCID 50 /0.1 ml for FPV, and 10 8.3 TCID 50 /0.1 ml for FHV-1.

Techniques: Real-time Polymerase Chain Reaction

Detection limit of conventional PCR. Templates of pMD18T-FPV (A) , pMD18T-FCV-1 (B) , and pMD18T-FHV-1 (C) were diluted by 10 times gradient to a dilution factor that could not be detected by conventional PCR. The detection limit was 5 × 10 3 copies/assay for FPV and 5 × 10 2 copies/assay for FCV and FHV-1. Template amount for curves 2–8 lanes was 5 × 10 7 -5 × 10 1 copies/assay. M, DL600 marker; NC, negative control.

Journal: Frontiers in Veterinary Science

Article Title: Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1

doi: 10.3389/fvets.2021.792322

Figure Lengend Snippet: Detection limit of conventional PCR. Templates of pMD18T-FPV (A) , pMD18T-FCV-1 (B) , and pMD18T-FHV-1 (C) were diluted by 10 times gradient to a dilution factor that could not be detected by conventional PCR. The detection limit was 5 × 10 3 copies/assay for FPV and 5 × 10 2 copies/assay for FCV and FHV-1. Template amount for curves 2–8 lanes was 5 × 10 7 -5 × 10 1 copies/assay. M, DL600 marker; NC, negative control.

Article Snippet: FCV (attenuated vaccine strain F9), FHV-1 (attenuated vaccine strain G2620A), and FPV (attenuated vaccine strain MW-1) were purchased from Intervet International B.V. Wild strains of these three viruses were isolated and stored by our laboratory, and the virus titers were 10 8.0 TCID 50 /0.1 ml for FCV, 10 7.0 TCID 50 /0.1 ml for FPV, and 10 8.3 TCID 50 /0.1 ml for FHV-1.

Techniques: Marker, Negative Control

Amplification and standard curves of the triplex assay. Serially diluted plasmids were mixed at equal concentration (from 5 × 10 7 copies/assay to 5 × 10 1 copies/assay) and used as template for RT-PCR. Amplification curves of the triplex assay for the detection of FPV (A) , FCV (C) , and FHV-1 (E) . The standard curves of FPV (B) , FCV (D) , and FHV-1 (F) were generated by plotting the Ct values (Y-axis) against the logarithm of copy numbers of plasmids (X-axis).

Journal: Frontiers in Veterinary Science

Article Title: Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1

doi: 10.3389/fvets.2021.792322

Figure Lengend Snippet: Amplification and standard curves of the triplex assay. Serially diluted plasmids were mixed at equal concentration (from 5 × 10 7 copies/assay to 5 × 10 1 copies/assay) and used as template for RT-PCR. Amplification curves of the triplex assay for the detection of FPV (A) , FCV (C) , and FHV-1 (E) . The standard curves of FPV (B) , FCV (D) , and FHV-1 (F) were generated by plotting the Ct values (Y-axis) against the logarithm of copy numbers of plasmids (X-axis).

Article Snippet: FCV (attenuated vaccine strain F9), FHV-1 (attenuated vaccine strain G2620A), and FPV (attenuated vaccine strain MW-1) were purchased from Intervet International B.V. Wild strains of these three viruses were isolated and stored by our laboratory, and the virus titers were 10 8.0 TCID 50 /0.1 ml for FCV, 10 7.0 TCID 50 /0.1 ml for FPV, and 10 8.3 TCID 50 /0.1 ml for FHV-1.

Techniques: Amplification, Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Generated

Intra- and inter-assay reproducibility of the triplex assay.

Journal: Frontiers in Veterinary Science

Article Title: Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1

doi: 10.3389/fvets.2021.792322

Figure Lengend Snippet: Intra- and inter-assay reproducibility of the triplex assay.

Article Snippet: FCV (attenuated vaccine strain F9), FHV-1 (attenuated vaccine strain G2620A), and FPV (attenuated vaccine strain MW-1) were purchased from Intervet International B.V. Wild strains of these three viruses were isolated and stored by our laboratory, and the virus titers were 10 8.0 TCID 50 /0.1 ml for FCV, 10 7.0 TCID 50 /0.1 ml for FPV, and 10 8.3 TCID 50 /0.1 ml for FHV-1.

Techniques: Inter Assay, Intra Assay

Detection of the co-infection models by triplex real-time PCR.

Journal: Frontiers in Veterinary Science

Article Title: Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1

doi: 10.3389/fvets.2021.792322

Figure Lengend Snippet: Detection of the co-infection models by triplex real-time PCR.

Article Snippet: FCV (attenuated vaccine strain F9), FHV-1 (attenuated vaccine strain G2620A), and FPV (attenuated vaccine strain MW-1) were purchased from Intervet International B.V. Wild strains of these three viruses were isolated and stored by our laboratory, and the virus titers were 10 8.0 TCID 50 /0.1 ml for FCV, 10 7.0 TCID 50 /0.1 ml for FPV, and 10 8.3 TCID 50 /0.1 ml for FHV-1.

Techniques: Virus, Real-time Polymerase Chain Reaction

Clinical samples detected by triplex assay and commercial kits.

Journal: Frontiers in Veterinary Science

Article Title: Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1

doi: 10.3389/fvets.2021.792322

Figure Lengend Snippet: Clinical samples detected by triplex assay and commercial kits.

Article Snippet: FCV (attenuated vaccine strain F9), FHV-1 (attenuated vaccine strain G2620A), and FPV (attenuated vaccine strain MW-1) were purchased from Intervet International B.V. Wild strains of these three viruses were isolated and stored by our laboratory, and the virus titers were 10 8.0 TCID 50 /0.1 ml for FCV, 10 7.0 TCID 50 /0.1 ml for FPV, and 10 8.3 TCID 50 /0.1 ml for FHV-1.

Techniques: